Recently, research on the application of artificial intelligence in the chemical process has been increasing rapidly. However, overfitting is a significant problem that prevents the model from being generalized well to predict unseen data on test data, as well as observed training data. Cross validation is one of the ways to solve the overfitting problem. In this study, the time-series cross validation method was applied to optimize the number of batch and epoch in the hyperparameters of the prediction model for the 2,3-BDO distillation process, and it compared with K-fold cross validation generally used. As a result, the RMSE of the model with time-series cross validation was lower by 9.06%, and the MAPE was higher by 0.61% than the model with K-fold cross validation. Also, the calculation time was 198.29 sec less than the K-fold cross validation method.